Composite

Part:BBa_K2549020:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)


LaG16-2-mN1c-GV2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
    Illegal XhoI site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2015
    Illegal SapI.rc site found at 1495


Design Notes

LaG16-2-mN1c-GV2 was assembled by joining LaG16-2 with a CD8α signal peptide and a Myc tag on its N terminal, mouse Notch1 core and GAL4-VP64 together using ClonExpress[1].


Source

This part was assembled by LaG16-2 (Part:BBa_K2446058), mouse Notch1 core (Part:BBa_K2549006), GAL4 DNA binding domain (Part:BBa_K801032), a linker (Part:BBa_K2549045) and VP64 transcription activator (Part:BBa_K1982012).


References

  1. ↑ http://www.vazymebiotech.com/products_detail/productId=195.html